Anti-Rabbit IgG, HRP-Linked Antibody
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Catalog number
6401-05
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Price
Please ask
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Size
500 µl
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Antibody Target
Anti-Rabbit IgG-HRP
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Target Alternative Name
Anti-Rabbit IgG, HRP-Linked Antibody, HRP linked secondary antibody
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Antibody Type
Polyclonal
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Species Reactivities
Rabbit
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.5 mg/ml
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Formulation
In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
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Application
Western blot, Immunohistochemistry, ELISA
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Application And Usages
Western blot analysis (1 µg/ml) and a 52 kDa band should be detected. However, the optimal conditions should be determined individually.
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Description
Goat anti-rabbit IgG (H+L), horseradish Peroxidase (HPR) conjugated. This antibody has been optimized as secondary antibody in chemiluminescent Western blotting applications
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
ILK, XGY1
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Short name
Anti-Rabbit IgG, -Linked Antibody
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Technique
Antibody, Rabbit, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, IgGs
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Host
Rabbit, Rabbits
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Isotype
Goat IgG, IgG
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Label
HRP
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Alternative name
Antibody tohost: rabbit Immunoglobulin G, horseradish peroxidase-Linked (antibody to-)
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Alternative technique
antibodies, rabbit-anti, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
integrin linked kinase
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
1997-05-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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