Target of this antibody:
Alternative names of antibody target:
ANGPT1; KIAA0003; Angiopoietin-1
Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a tyrosine-kinase receptor expressed primarily on vascular endothelial cells and early hematopoietic cells. Ang-1/ Tie-2 signaling promotes angiogenesis during the development, remodeling, and repair of the vascular system. Transgenic mice lacking expression of either Ang-1 or Tie-2 fail to develop a fully functional cardiovascular system and die before birth. Postnatally, the angiogenic activity of Ang-1/Tie-2 is required during normal tissue repair and remodeling of the female endometrium in the menstrual cycle. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 (Ang-2), a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules, Ang-1 enhances endothelial cell survival and capillary morphogenesis, and lessens capillary permeability. As such, Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders, including several severe human pulmonary diseases. The efficacy of cell-based Ang-1 gene therapy for acute lung injury (ALI) has recently been studied in a rat model of ALI. The results of this study show that such therapy can markedly improve lung condition and suggest that Ang-1 therapy may represent a potential new strategy for the treatment and/or prevention of acute respiratory distress injury (ARDI), a significant cause of morbidity and mortality in critically ill patients. Recombinant human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.
Clonality of antibody:
This antibody reacts with:
Human. Predicted cross reactivity with mouse, bovine and pig samples.
This ANGPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the C-terminal region of human ANGPT1.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
This antibody is purified through a protein A column, followed by peptide affinity purification.
This antibody has been succesfully tested for use in following methods:
Western blot: ~1:1000.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anti-ANGPT1 Antibody (CT)
antibody to-angiopoietin 1 (Antibody to) (CT)
Alternative to gene target
angiopoietin 1, AGP1 and AGPT and ANG1, ANGPT1 and IDBG-32292 and ENSG00000154188 and 284, receptor tyrosine kinase binding, Extracellular, Angpt1 and IDBG-137812 and ENSMUSG00000022309 and 11600, ANGPT1 and IDBG-644947 and ENSBTAG00000014051 and 282140