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Clone
Tsp232A
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Immunogen
Recombinant tsp23
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Antibody s full description
Mouse Anti-Human TSP23 Monoclonal IgG1 Kappa Antibody, Clone: Tsp232A: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Transcript similar p23 Antibody, AARSD1 Antibody, AASD1_HUMAN Antibody, Alanyl-tRNA editing protein Aarsd1 Antibody, Alanyl-tRNA synthetase domain containing 1 Antibody, Alanyl-tRNA synthetase domain-containing protein 1 Antibody, MGC2744 Antibody, OTTHUMP00000204017 Antibody, OTTHUMP00000204019 Antibody, OTTHUMP00000204020 Antibody
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Raised in
Mouse
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Antibody s target
TSP23
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Primary research fields
Cancer, Heat Shock, Cell Signaling , Epigenetics
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Brandname
None
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Antibodies applications
WB, IHC, AM
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Antibody s reactivity
Human, Mouse
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Antibody s dilutions
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~19kDa. Detects Tsp23, no cross-reactivity to p23.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-194 was sufficient for detection of tsp23 in 20 µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology. Specifically TSP23 (transcript similar p23) displays 44% and 17% amino acid identity with p23 and Sba1p respectively (10).
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Bibliography
1. Johnson J.L., Beito T. G., Krco C.J., Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K., Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A., Toft D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D., Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J., Toft D.O. (2003) Cell Stress Chaperones.8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia 10. Freeman B.C., Felts S.J., Toft D.O., Yamamato K.R. (2000) Genes Dev. 14(4): 433-434.
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Released date
5-May-2011
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001136126.1
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Gene number
80755
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Protein number
B9A003
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (SMC-194). Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (SMC-194) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (SMC-194). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (SMC-194) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Tsp23 antibody [TSp232A] used in Immunohistochemistry (IHC) on Mouse Heart Skeletal Muscle (SMC-194) Mouse Anti-Tsp23 Antibody [TSp232A] used in Western Blot (WB) on Human Heat Shocked HeLa cell lysates (SMC-194)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
