Human recombinant UBE2E2 (UbcH8)

• Catalog number
  6438-3
• Price
  Please ask
• Size
  3 nmol

• Synonyms
  Human recombinant UBE2E2 (UbcH8)
• Alternative_names
  RIG-B, UBCH8, MGC40331, UBE2L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, Ubiquitin-protein ligase L6, Ubiquitin carrier protein L6, and Retinoic acid-induced gene B protein.
• Description
  Ubiquitin conjugating enzyme involved in conjugation of ubiquitin to target substrates.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Biological activity
  Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.
• Molecular Weight
  22.255 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol.
• Background Information
  Ubiquitin-conjugating enzyme E2 (UBE2E2) is a protein that in humans is encoded by the UBE2E2 gene. Amino acid sequence within the UBC domain of UBE2E2 shares over 90% identity with human UbcH6, mouse UbcM2, and Drosophila UbcD2, whereas the N-terminal region shows little amino acid sequence similarity with known proteins. The UBE2E2 protein forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UBE2E2   UbcH8  
• Gene symbol
  UBE2E2-DT, UBE2E2
• Short name
  recombinant UBE2E2 (UbcH8)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. UBE2E2 (UbcH8)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:44512
• Gene
  UBE2E2-DT
• Long gene name
  UBE2E2 divergent transcript
• Synonyms gene
  • UBE2E2-AS1
• Synonyms gene name
  • UBE2E2 antisense RNA 1 (head to head)
• Locus
  3p24.3
• Discovery year
  2012-11-13
• Entrez gene record
  100505877
• RefSeq identity
  • NR_136176
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000155608

Gene info

• Identity
  HGNC:12478
• Gene
  UBE2E2
• Long gene name
  ubiquitin conjugating enzyme E2 E2
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
  • ubiquitin-conjugating enzyme E2E 2
• Synonyms
  • UbcH8
  • FLJ25157
• GenBank acession
  • AK057886
• Locus
  3p24.3
• Discovery year
  1997-12-15
• Entrez gene record
  7325
• Pubmed identfication
  • 9371400
• RefSeq identity
  • NM_152653
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000130482

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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