Human recombinant UBE2R1 (CDC34)

• Catalog number
  6436-3
• Price
  Please ask
• Size
  3 nmol

• Synonyms
  Human recombinant UBE2R1 (CDC34)
• Alternative_names
  Human recombinant UBE2R1 (CDC34), CDC-34, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, E2-CDC34, CDC34, EC 6.3.2.19, UBC3.
• Description
  Ubiquitin conjugating enzyme involved in conjugation of ubiquitin to target substrates.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Biological activity
  Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.
• Molecular Weight
  27.730 kDa with His Tag.
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.
• Background Information
  UBE2R1 is important in the control of cell cycle and DNA replication. In association with different E3 complexes, including SCF, it has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. UBE2R1 substrates include B-Myb, Wee1, MyoD, ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of UBE2R1 by virtue of their SCF requirement for proteolysis. This protein is useful for in vitro ubiquitinylation reactions.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UBE2R1   CDC34  
• Gene symbol
  CDC34
• Short name
  recombinant UBE2R1 (CDC34)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. UBE2R1 (CDC34)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1734
• Gene
  CDC34
• Long gene name
  cell division cycle 34, ubiqiutin conjugating enzyme
• Synonyms
  • E2-CDC34
  • UBE2R1
  • UBC3
• Synonyms name
  • ubiquitin conjugating enzyme E2 R1
• GenBank acession
  • L22005
• Locus
  19p13.3
• Discovery year
  1994-05-12
• Entrez gene record
  997
• Pubmed identfication
  • 8248134
  • 16210246
• RefSeq identity
  • NM_004359
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000180558

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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