Human recombinant UBE2L3

• Catalog number
  6431-3
• Price
  Please ask
• Size
  3 nmol

• Synonyms
  Human recombinant UBE2L3
• Alternative_names
  Ubiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3, Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, UbcM4.
• Description
  Ubiquitin conjugating enzyme involved in conjugation of ubiquitin to target substrates.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Biological activity
  Typical enzyme concentration of 100 nM - 1 mM is used for in vitro conjugation, depending on conditions.
• Molecular Weight
  17.861 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.
• Background Information
  UBE2L3 (UbcH7) is an E2 (ubiquitin conjugating enzyme) ligase involved in the conjugation of ubiquitin to target substrates, along with E1 and E3 enzymes. UbcH7 has been shown to work with HECT domain E3’s (E6-AP), in addition to RING containing E3’s. UbcH7 is homologous to the UbcH5 family.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UBE2L3  
• Gene symbol
  UBE2L3
• Short name
  recombinant UBE2L3
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. UBE2L3
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12488
• Gene
  UBE2L3
• Long gene name
  ubiquitin conjugating enzyme E2 L3
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2L 3
• Synonyms
  • UBCH7
• GenBank acession
  • AJ000519
• Locus
  22q11.21
• Discovery year
  1997-03-21
• Entrez gene record
  7332
• Pubmed identfication
  • 8672131
  • 9693040
• RefSeq identity
  • NM_198157
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000150823

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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