Human recombinant UBE2D3

• Catalog number
  6430-3
• Price
  Please ask
• Size
  3 nmol

• Synonyms
  Human recombinant UBE2D3
• Alternative_names
  Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-protein ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2-17 kDa 3, UBC4/5, UBCH5C, MGC5416, MGC43926, UBE2D3.
• Description
  Ubiquitin conjugating enzyme involved in conjugation of ubiquitin to target substrates.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Biological activity
  Typical enzyme concentration of 100 nM - 1 mM is used for in vitro conjugation, depending on conditions.
• Molecular Weight
  28.707 kDa with a His6SUMO Tag
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  75 µL of a 40 µM solution (3 nmoles) in 25 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.
• Background Information
  UBE2D3 (UbcH5b) is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. The UbcH5 family (including UbcH5a and UbcH5c, having 88% and 89% sequence identity with UbcH5b respectively) are the human homologues of the Saccharomyces cerevisiae UBC4/5 family (~79% sequence identity). Members of the UbcH5 family have been implicated in a number of inflammatory and cancer pathways including the ubiquitylation of p53 and IκBα with the help of different E3 ligases (Hdm2 and SCF complexes respectively).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UBE2D3  
• Gene symbol
  UBE2D3-AS1, UBE2D3
• Short name
  recombinant UBE2D3
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. ubiquitin-conjugating enzyme E2D 3
• Alternative technique
  rec
• Alternative to gene target
  ubiquitin-conjugating enzyme E2D 3, E2(17)KB3 and UBC4/5 and UBCH5C, UBE2D3 and IDBG-32113 and ENSG00000109332 and 7323, acid-amino acid ligase activity, nuclei, Ube2d3 and IDBG-269073 and ENSMUSG00000078578 and 66105, IDBG-639940 and IDBG-639940 and ENSBTAG00000045753 and

Gene info

• Identity
  HGNC:54095
• Gene
  UBE2D3-AS1
• Long gene name
  UBE2D3 antisense RNA 1
• Locus
  4q24
• Discovery year
  2018-11-04
• Entrez gene record
  105377348
• RefSeq identity
  • NR_131185
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:12476
• Gene
  UBE2D3
• Long gene name
  ubiquitin conjugating enzyme E2 D3
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
  • ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
  • ubiquitin-conjugating enzyme E2D 3
• Synonyms
  • UbcH5C
• GenBank acession
  • U39318
• Locus
  4q24
• Discovery year
  1997-03-21
• Entrez gene record
  7323
• Pubmed identfication
  • 8530467
• RefSeq identity
  • NM_181893
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000131119

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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