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Immunogen
Synthesized peptide derived from internal of human HMGB1.
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Antibody s target
Human HMGB1
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Antibody s full description
Rabbit Anti-Human HMGB1 Polyclonal
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Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Domain Families, Hmg box
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Amphoterin Antibody, Chromosomal protein nonhistone HMG1 Antibody, High mobility group 1 Antibody, High mobility group protein B1 Antibody, HMG-1 Antibody, HMG3 Antibody, HMGB1 Antibody, HMGB1_HUMAN Antibody, SBP 1 Antibody, Sulfoglucuronyl carbohydrate binding protein Antibody
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Verified applications
WB, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total HMGB1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1259 was sufficient for detection of HMGB1 in 10 µg of Jurkat, HepG2, COLO, Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Chromosome, Cytoplasm, Endosome
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Tissue specificity
Ubiquituous. Expressed in platelets
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Scientific context
HMGB1, also known as high mobility group box 1 protein, and functions much like histones as they are important chromatin proteins. In the nucleus, HMGB1 interacts with nucleosomes, transcription factors and histones. The presence of HMGB1 in the nucleus depends on postranslational modifications. Hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 is also secreted by immune cells and acts as a cytokine mediator of inflammation. The mechanism of inflammation and damae is its binding to TLR2. HMGB1 also interacts with P53 and has been proposed as a target for cancer therapy.
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Bibliography
1. Bianchi M.E., Agresti A. (2005). Curr. Opin. Genet. Dev. 15(5): 496–506. 2. Klune J.R., Dhupar R., Cardinal J., Billiar T.R., Tsung A. (2008). Mol. Med. 14(7-8): 476–84. 3. Imamura T., Izumi H., Nagatani G., Ise T., Nomoto M., Iwamoto Y., Kohno K. (2001). J. Biol. Chem. 276(10): 7534–40. 4. Lotze M.T., DeMarco R.A. (2003). Curr Opin Investig Drugs. 4(12): 1405–9.
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Released date
6/Sep/2016
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NCBI number
NP_002119.1
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Gene number
3146
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Protein number
P09429
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human Jurkat, HepG2, COLO, Hek293 cell lysates showing detection of ~25kDa HMGB1 protein using Rabbit Anti-HMGB1 Polyclonal Antibody (SPC-1259). Lane 1: Human Jurkat. Lane 2: Human HepG2. Lane 3: Human COLO. Lane 4: Hek293. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-HMGB1 Polyclonal Antibody (SPC-1259) at 1:1000. Predicted/Observed Size: ~25kDa. Rabbit Anti-HMGB1 Antibody used in Western blot (WB) on Jurkat, HepG2, COLO, Hek293 cell lysates (SPC-1259)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
HMGB1
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Short name
Rabbit Anti- HMGB1 Polyclonal: ATTO 633
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 633
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman high mobility group box 1 polyclonal: ATTO 633
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
high mobility group box 1, HMG1 and HMG3 and SBP-1, HMGB1 and IDBG-21100 and ENSG00000189403 and 3146, DNA binding, nuclei, Hmgb1 and IDBG-209311 and ENSMUSG00000066551 and 100862258,102642363,15289,619937, HMGB1 and IDBG-630533 and ENSBTAG00000018103 and 282691
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