MOUSE Anti-HUMAN B CELLS:FITC Antibody

  • Catalog number
    GEN211102
  • Price
    Please ask
  • Size
    100 Tests
  • Also known as
    B CELLS
  • Other names
    B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20
  • Gene name synonims
    MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20
  • Other gene names
    MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgM
  • Clone
    FMC7
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    B CELLS This item recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    FITC (Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
  • Concentration
    N/A
  • Storage and shipping
    Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    MBS Monoclonals supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
    CELLS  
  • Gene symbol
    GCM2, IKBKGP1, LAT2
  • Short name
    MOUSE Anti- B CELLS:FITC Antibody
  • Technique
    anti-Human, Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Fluorescein, mouses
  • Host
    mouse
  • Label
    FITC
  • Species
    Human, Humans, Mouses
  • Alternative name
    Anti-MOUSE ANTI HUMAN B CELLS:FITC
  • Alternative technique
    antibodies, murine, fluorescine
  • Tissue
    cells
Gene info
  • Identity
  • Gene
  • Long gene name
    glial cells missing transcription factor 2
  • Synonyms gene
  • Synonyms gene name
    • glial cells missing (Drosophila) homolog b
    • glial cells missing homolog 2 (Drosophila)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-10-02
  • Entrez gene record
  • Pubmed identfication
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    inhibitor of nuclear factor kappa B kinase subunit gamma pseudogene 1
  • Synonyms gene
  • Synonyms gene name
    • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma pseudogene
    • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma pseudogene 1
  • Synonyms
  • Synonyms name
  • Locus
  • Discovery year
    2007-07-23
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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