Amplite Colorimetric Peroxidase (HRP) Assay Kit *Blue Color* is a chromogenic peroxidase substrate, it is much more sensitive to both H2O2 and peroxidase than other chromogenic peroxidase substrates such as TMB, ABTS, OPD and K-Blue.The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors.
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
aat supplies other types of Assays as 1.
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.Colorimetric assays or detection use UV absorption or enzymatic color reaction.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Blue Substrate,HRP Conjugated
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH