Amplite Colorimetric Peroxidase Assay Kit *Blue Color* is a ready to use kit which is designed to save considerably more assay time when compared to the alternative indoor methods. The easy to follow and user friendly protocol will guide you through each step.
The liquid components of the Amplite Colorimetric Peroxidase Assay Kit *Blue Color* may get entrapped on the walls and seal of the tubes. Before starting the assay briefly spin the vials on a centrifuge to ensure that all of the content is on the bottom. Avoid cycles of freezing and thawing as they may denaturate protein components thus reducing the kits performance. Some of the components of the Amplite Colorimetric Peroxidase Assay Kit *Blue Color* may be photosensitive, therefore exposing them to bright light should be avoided.
aat supplies other types of Assays as 1.
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH