CD229 (CDABP0070, Cell Surface Molecule Ly9, hLy9, Lymphocyte Antigen 9, Ly 9, Ly9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface Antigen Ly9 Precursor) (FITC)
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Catalog number
MBS632688
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Price
Please ask
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Size
5x0.1 mg
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Products_type
Antibody
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Products_short_name
[CD229]
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Products_name_syn
[Anti -CD229 (CDABP0070, Cell Surface Molecule Ly9, hLy9, Lymphocyte Antigen 9, Ly 9, Ly9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface Antigen Ly9 Precursor) (FITC)]
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Other_names
[cytochrome oxidase subunit I]
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Clonality
Monoclonal
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Clone
[HLy9.1.25]
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Reactivity
Human
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Specificity
Recognizes human CD229at ~100-120kD. Species sequence homology: mouse.
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Purity
Purified Purified
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Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
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Tested_application
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
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Description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits. Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
LY9
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Short name
CD229 (CDABP0070, Surface Molecule Ly9, hLy9, Lymphocyte Antigen 9, Ly 9, Ly9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface Antigen Ly9 ) (FITC)
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Technique
antigen, FITC, antigenes, Fluorescein
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Alternative name
CD229 (CDABP0070, cellular Surface Molecule lymphocyte antigen 9, hLy9, Lymphocyte protein 9, Ly 9, lymphocyte antigen 9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface protein lymphocyte antigen 9 Precursor) (fluorecein)
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Alternative technique
antigenes, fluorescine
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Alternative to gene target
lymphocyte antigen 9, CD229 and hly9 and mLY9 and SLAMF3, LY9 and IDBG-104095 and ENSG00000122224 and 4063, protein binding, Plasma membranes, Ly9 and IDBG-204729 and ENSMUSG00000004707 and 17085, LY9 and IDBG-630402 and ENSBTAG00000002947 and 528926
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Tissue
cell, lymphocyte
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Gene info
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Identity
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Gene
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Long gene name
lymphocyte antigen 9
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-06-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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