HMGB1 Polyclonal Antibody
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Catalog number
CAC07822
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Price
Please ask
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Size
50 μg
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Other name
High mobility group protein B1,High mobility group protein 1,HMG-1,HMGB1,HMG1
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Immunogen
Recombinant Human High mobility group protein B1 protein (2-215AA)
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Verified reactivity
Human, Mouse
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Verified applications
ELISA,WB,IHC,IF. Recommended dilution:WB:1:500-1:5000,IHC:1:20-1:200,IF:1:50-1:200
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Raised in
Rabbit
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Clonality
Polyclonal
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Protein number
P09429
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Gene number
Please refer to GenBank
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NCBI number
Please refer to NCBI
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Purity
Protein G purified, >95%
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Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
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Use before
1 year
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Shipping requirements
Blue ice
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Estimated production time
3-7 business days
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Supplementary information
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).
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Notes
For research use only. Not for diagnostic procedures.
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Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
HMGB1
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Short name
HMGB1 Polyclonal Antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
Unconjugated
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Alternative name
high mobility group box 1 polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
high mobility group box 1, HMG1 and HMG3 and SBP-1, HMGB1 and IDBG-21100 and ENSG00000189403 and 3146, DNA binding, nuclei, Hmgb1 and IDBG-209311 and ENSMUSG00000066551 and 100862258,102642363,15289,619937, HMGB1 and IDBG-630533 and ENSBTAG00000018103 and 282691
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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