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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr653 and Tyr654 of human FGFR1 (AA650-657)
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Antibody s target
Human FGFR1 (pTyr653 + pTyr654)
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Antibody s full description
Rabbit Anti-Human FGFR1 (pTyr653 + pTyr654) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Basic fibroblast growth factor receptor 1 Antibody, bFGF-R-1 Antibody, BFGFR Antibody, FGFR1 Antibody, CD331 Antibody, CEK Antibody, FGFBR Antibody, FGFR1 Antibody, FGFR1/PLAG1 fusion Antibody, fibroblast growth factor receptor 1 Antibody, FLG Antibody, FLT2 Antibody, Fms-like tyrosine kinase 2 Antibody, HBGFR Antibody, heparin-binding growth factor receptor Antibody, HH2 Antibody, HRTFDS Antibody, hydroxyaryl-protein kinase Antibody, KAL2 Antibody, N-SAM Antibody, OGD Antibody, Proto-oncogene c-Fgr Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 91.868 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-981 was sufficient for detection of FGFR1 (pTyr653 + pTyr654) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Nucleus, Cytoplasm, Cytosol, Cytoplasmic Vesicle
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Tissue specificity
Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines.
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Scientific context
FGFR1 is a receptor-tyrosine kinase that is activated upon binding fibroblast growth factor (FGF). It is a key player in many numerous cell signalling pathways including MAPK/AKT/PLC/STAT/NF-kB. FGF and the FGFR1 is a stimulator of cell differentiation, proliferation, repair, angiogenesis, pro-survival, anti-apoptosis, etc. Overexpression leads to disregulated cell growth which forms the basis of early oncogenesis. This occurs through amplification, gene duplication, or translocation leading to constitutive activation of the receptor.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_075598
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Gene number
2242
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Protein number
P11362
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
APC, FGFR1
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Short name
Rabbit Anti- FGFR1 (pTyr653 + pTyr654) Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman fibroblast growth factor receptor 1 (pTyr653 + pTyr654) polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
fibroblast growth factor receptor 1, bFGF-R-1 and BFGFR and CD331 and CEK and FGFBR and FGFR-1 and FLG and FLT-2 and FLT2 and HBGFR and HH2 and HRTFDS and KAL2 and N-SAM and OGD, FGFR1 and IDBG-17796 and ENSG00000077782 and 2260, transferase activity, nuclei, Fgfr1 and IDBG-143767 and ENSMUSG00000031565 and 14182, FGFR1 and IDBG-629273 and ENSBTAG00000015457 and 281768
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