Anti-Rat IgG (H+L)-FITC

  • Catalog number
    MBS674910
  • Price
    Please ask
  • Size
    1000ug
  • Alternative name1
    Anti-Rabbit Anti-Rat IgG (H+L)-FITC
  • Alternative name2
    Rabbit Anti-Rat IgG (H+L)-FITC
  • Alternative name3
    Lapin Anti-Rattus norvegicus IgG (H+L)-FITC
  • Alternative name4
    Rabbit Anti-Rat IgG (H+L)
  • Alternative name5
    Rabbit Anti-Rat IgG(H+L) - FITC; Rabbit Anti-Rat IgG (H+L)
  • Other names
    N/A
  • Gene name
    N/A
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Category
    Secondary Antibodies
  • Clonality
    Polyclonal
  • Immunoglobulin isotype
    IgG
  • Clone
    Polyclonal antibody
  • Host organism
    Rabbit
  • Species reactivity
    Rat
  • Specificity and cross reactivity
    Reacts with the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c, and with the light chains of rat IgM as demonstrated by ELISA and/or flow cytometry.
  • Purification method
    Affinity chromatography on pooled rat IgG covalently linked to agarose.
  • Form Appearance
    Fluorescein (FITC) Conjugate
  • Concentration
    N/A
  • Storage and shipping
    The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at The items should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
  • Tested for
    ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
  • Description
    productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
  • Advisory
    In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    H+L  
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    Anti- IgG (H+L)-FITC
  • Technique
    anti-, anti, FITC, IgG, antibody to, antibodies, Fluorescein, IgGs
  • Host
    Rat
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Species
    Rat, Rats
  • Alternative name
    Antibody toRat Immunoglobulin G (heavy + light chain)-fluorecein
  • Alternative technique
    antibodies, fluorescine, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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