Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

• Catalog number
  K444-100
• Price
  Please ask
• Size
  100 assays

• Description
  Sensitive Fluorometric Assay to Detect Gelatinase in Mammalian Samples
• Summary
  • Detection method- Fluorometric (490/520 nm) • Species reactivity- mammalian • Applications- - Measurement of gelatinase activity - For screening/studying/characterizing gelatinase inhibitors
• Detection Method
  Fluorescence (490/520 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of gelatinase activity • For screening/studying/characterizing gelatinase inhibitors
• Sample Type
  Purified Gelatinase, cell lysates, tissues lysates
• Features Benefits
  • Rapid & sensitive • Simple assay to measure gelatinase activity as well as to screen/study/characterize potential inhibitors of gelatinase • Includes Fluorometric Gelatinase Substrate and Positive Control
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells. Gelatinase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for gelatinase activity include gelatin Zymography where samples are electrophoresed on a gelatin-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. BioVision’s Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU for bacterial collagenase) in biological samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Gelatinase   Gelatin   Degradation/Zymography   Kit   Fluorometric  
• Short name
  Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Gelatinase (Gelatin Degradation/Zymography) test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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