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Available ordering format
Lyophilized
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Immunogen
GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 122 | anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranesAS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membraneCollection of antibodies to Chlamydomonas proteins
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Recommended dilutions for use
1 : 1 000 with alkaline phosphatase (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below
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References
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.
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Scientific context
Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.. Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.
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Notes
cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii
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Protein number
A8J9G6
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TAIR number
Refer to NCBI
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