Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric)

• Catalog number
  K949-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Fluorescence (Ex/Em= 490/480 nm) • Species reactivity- Mammalian • Application- This assay has a detection sensitivity as low as 0.1 mU.
• Detection Method
  Fluorescence (Ex/Em= 490/480 nm)
• Species Reactivity
  Mammalian
• Applications
  This assay has a detection sensitivity as low as 0.1 mU.
• Sample Type
  Cell and tissue culture lysates and purified enzyme preparations.
• Features Benefits
  • Simple procedure • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Protein Disulfide Isomerases (PDI, EC: 5.3.4.1) are multifunctional proteins that constitute a thio-disulfide oxidoreductase family. PDI is abundant in the lumen of endoplasmic reticulum (ER). PDI plays an essential role in catalyzing the rearrangement of disulfide (S-S) bonds in proteins and functions as a chaperone by inhibiting protein aggregation. Recent studies show that PDI activity is essential for cancer cell survival and proliferation, and targeting PDI activity with specific inhibitors abrogates survival responses leading to ER stress in cancer cells. Therefore, the detection of PDI activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purposes. BioVision’s PDI Activity Assay kit utilizes the reductase activity PDI for determining its activity in a variety of samples. The Kit employs a synthetic quenched fluorescent substrate which gets cleaved in the presence of PDI producing an enhanced fluorometric signal (Ex/Em = 490/580 nm). In the presence of BioVision’s PDI specific inhibitor, the quenched fluorescent substrate cannot be cleaved by PDI. BioVision’s PDI Activity Assay Kit is simple, sensitive, and high-throughput adaptable with the detection limit as low as 0.1 mU.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Protein   Disulfide   Isomerase   PDI   Activity   Kit   Fluorometric  
• Gene symbol
  PDIA2, PADI1, ERP29, P4HB
• Short name
  Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Protein Disulfide Isomerase (PDI) Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:14180
• Gene
  PDIA2
• Long gene name
  protein disulfide isomerase family A member 2
• Synonyms gene
  • PDIP
• Synonyms gene name
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide isomerase family A, member 2
• Synonyms
  • PDA2
  • PDI
  • PDIR
• GenBank acession
  • U19948
• Locus
  16p13.3
• Discovery year
  2000-12-21
• Entrez gene record
  64714
• Pubmed identfication
  • 8561901
• RefSeq identity
  • NM_006849
• Classification
  • Protein disulfide isomerases
• VEGA ID
  OTTHUMG00000064891

Gene info

• Identity
  HGNC:18367
• Gene
  PADI1
• Long gene name
  peptidyl arginine deiminase 1
• Synonyms gene name
  • peptidyl arginine deiminase, type I
• Synonyms
  • HPAD10
  • PDI1
  • PDI
  • PAD1
• Synonyms name
  • peptidylarginine deiminase type I
  • protein-arginine deiminase type-1
  • hPAD-colony 10
• GenBank acession
  • AB033768
• Locus
  1p36.13
• Discovery year
  2002-03-12
• Entrez gene record
  29943
• Pubmed identfication
  • 12416996
• RefSeq identity
  • NM_013358
• Classification
  • Peptidyl arginine deiminases
• VEGA ID
  OTTHUMG00000002294

Gene info

• Identity
  HGNC:13799
• Gene
  ERP29
• Long gene name
  endoplasmic reticulum protein 29
• Synonyms gene
  • C12orf8
• Synonyms gene name
  • chromosome 12 open reading frame 8
• Synonyms
  • ERp28
  • ERp31
  • PDI-DB
  • PDIA9
• Synonyms name
  • protein disulfide isomerase family A, member 9
• GenBank acession
  • X94910
• Locus
  12q24.13
• Discovery year
  2001-04-27
• Entrez gene record
  10961
• Pubmed identfication
  • 9738895
  • 9037184
• Classification
  • Protein disulfide isomerases
• VEGA ID
  OTTHUMG00000169637

Gene info

• Identity
  HGNC:8548
• Gene
  P4HB
• Long gene name
  prolyl 4-hydroxylase subunit beta
• Synonyms gene
  • PO4DB
  • ERBA2L
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1)
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
  • prolyl 4-hydroxylase, beta polypeptide
• Synonyms
  • PDIA1
  • PROHB
  • DSI
  • GIT
  • PDI
  • PO4HB
  • P4Hbeta
• Synonyms name
  • protein disulfide isomerase-associated 1
  • protein disulfide isomerase family A, member 1
  • collagen prolyl 4-hydroxylase beta
• GenBank acession
  • J02783
• Locus
  17q25.3
• Discovery year
  1986-01-01
• Entrez gene record
  5034
• Pubmed identfication
  • 8111381
• RefSeq identity
  • NM_000918
• Classification
  • Protein disulfide isomerases
• VEGA ID
  OTTHUMG00000150269

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