Protein G
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Catalog number6510-1000
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PricePlease ask
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Size1 gram
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SynonymsProtein G
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Alternative_namesProtein G
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DescriptionGenetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Binding CapacityUnder optimal conditions, 1 mg protein G will bind approximately 5 mg human IgG. Optimal binding of Protein G to antibodies occurs at pH 5.0 to 6.0 and can be eluted over a pH range of 2.5 to 3.0.
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Molecular Weight26.1 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized with no additives
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Reconstitution InstructionsReconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
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Background InformationProtein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolGPR88, GPR33, GPR173
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Short nameProtein G
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Alternative nameProtein G
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Gene info
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Identity
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Gene
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Long gene nameG protein-coupled receptor 88
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Synonyms gene name
- G-protein coupled receptor 88
- G protein coupled receptor 88
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GenBank acession
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Locus
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Discovery year2000-06-02
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Entrez gene record
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RefSeq identity
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Classification
- G protein-coupled receptors, Class A orphans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameG protein-coupled receptor 33
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Synonyms gene name
- G protein-coupled receptor 33, pseudogene
- G protein-coupled receptor 33 pseudogene
- G protein-coupled receptor 33
- G protein-coupled receptor 33 (gene/pseudogene)
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GenBank acession
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Locus
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Discovery year1997-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein-coupled receptors, Class A orphans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameG protein-coupled receptor 173
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Synonyms gene name
- G-protein coupled receptor 173
- G protein coupled receptor 173
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-08-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein-coupled receptors, Class A orphans
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VEGA ID
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Locus Specific Databases