Protein G

• Catalog number
  6510-10
• Price
  Please ask
• Size
  10 mg

• Synonyms
  Protein G
• Alternative_names
  Protein G
• Description
  Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Binding Capacity
  Under optimal conditions, 1 mg protein G will bind approximately 5 mg human IgG. Optimal binding of Protein G to antibodies occurs at pH 5.0 to 6.0 and can be eluted over a pH range of 2.5 to 3.0.
• Molecular Weight
  26.1 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
• Background Information
  Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet

• Gene target
  Protein  
• Gene symbol
  GPR88, GPR33, GPR173
• Short name
  Protein G
• Alternative name
  Protein G

Gene info

• Identity
  HGNC:4539
• Gene
  GPR88
• Long gene name
  G protein-coupled receptor 88
• Synonyms gene name
  • G-protein coupled receptor 88
  • G protein coupled receptor 88
• GenBank acession
  • AB042411
• Locus
  1p21.2
• Discovery year
  2000-06-02
• Entrez gene record
  54112
• RefSeq identity
  • NM_022049
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000010981

Gene info

• Identity
  HGNC:4489
• Gene
  GPR33
• Long gene name
  G protein-coupled receptor 33
• Synonyms gene name
  • G protein-coupled receptor 33, pseudogene
  • G protein-coupled receptor 33 pseudogene
  • G protein-coupled receptor 33
  • G protein-coupled receptor 33 (gene/pseudogene)
• GenBank acession
  • AF045767
• Locus
  14q12
• Discovery year
  1997-06-12
• Entrez gene record
  2856
• Pubmed identfication
  • 9653656
  • 15987686
• RefSeq identity
  • NM_001197184
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000154921

Gene info

• Identity
  HGNC:18186
• Gene
  GPR173
• Long gene name
  G protein-coupled receptor 173
• Synonyms gene name
  • G-protein coupled receptor 173
  • G protein coupled receptor 173
• Synonyms
  • SREB3
• GenBank acession
  • AB040801
• Locus
  Xp11.22
• Discovery year
  2004-08-19
• Entrez gene record
  54328
• Pubmed identfication
  • 10833454
• RefSeq identity
  • NM_018969
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000021596
• Locus Specific Databases
  • Global Variome shared LOVD

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