taq hotstart - 1000 units w/reaction buffer (mgcl2) [di]
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Catalog number
IB43203
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Price
Please ask
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Size
1000 UNITS
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Description
Buffering solutions are useful to keep the pH range sable when using this reagent of IBI Scientific. Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. it can be antibody based or chemical blocked. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity.
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Gene target
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Short name
taq - 1000 units w/ (mgcl2) [di]
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Technique
Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. IBI Scientific TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name
taq hotstart - 1000 units w/reaction solution (mgcl2) [di]
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