Human CellExp™ CD58 /LFA-3, human recombinant
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Catalog number
7427-10
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Price
Please ask
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Size
10 ug
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Synonyms
CD58, LFA3, Ag3
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Alternative_names
CD58, LFA3, Ag3
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured in a cell proliferation assay using human CD3+ T cells in the presence of anti-CD3. The ED50 for this effect is typically 1 - 5 µg/ml in the presence of anti-CD3 immobilized at 25 ng/ml.
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Results
Measured in a cell proliferation assay using human CD3+ T cells in the presence of anti-CD3. The ED50 for this effect is typically 1 - 5 µg/ml in the presence of anti-CD3 immobilized at 25 ng/ml.
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22.3 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 30-45 kDa due to different glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD58, ITGAL, ITGB2, MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11
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Short name
CellExp™ CD58 /LFA-3, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD58 molecule /LFA-3, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
CD58 molecule, ag3 and LFA-3 and LFA3, CD58 and IDBG-101394 and ENSG00000116815 and 965, protein binding, Cell surfaces, CD58 and IDBG-634482 and ENSBTAG00000040131 and 782186
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Gene info
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Identity
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Gene
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Long gene name
CD58 molecule
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Synonyms gene
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Synonyms gene name
- CD58 antigen, (lymphocyte function-associated antigen 3)
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GenBank acession
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Locus
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Discovery year
1989-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
integrin subunit alpha L
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Synonyms gene
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Synonyms gene name
- integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Integrin alpha subunits
- CD molecules
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
integrin subunit beta 2
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Synonyms gene
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Synonyms gene name
- integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
- integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Integrin beta subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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