Follistatin, human recombinant
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Catalog number
4708-100
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Price
Please ask
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Size
100 ug
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Synonyms
FST, FS, Activin-binding protein
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Alternative_names
FST, FS, Activin-binding protein
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Description
An autocrine glycoprotein involved in binding and bioneutralization of members of the TGF-β superfamily
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED₅₀ is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.
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Results
0.1-0.4 µg/ml
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Molecular Weight
31.5 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl
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Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background Information
Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-β ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-β1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
FST, FSTL1, FSTL3
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Short name
Follistatin, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Follistatin, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
follistatin
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
follistatin like 1
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- SPARC family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
follistatin like 3
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Synonyms gene name
- follistatin-like 3 (secreted glycoprotein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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