Anti-DFFB (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-0043R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  DFFB
• Specificity
  This antibody reacts specifically with DFFB
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm, Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from mouse DFF-40 beta
• Gene ID
  13368
• Swiss Prot
  O54788
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40; Dffb
• Also known as
  DFFB Polyclonal Antibody
• Other name
  Anti-DFFB Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  DFFB  
• Gene symbol
  DFFB
• Short name
  Anti-DFFB
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  DFFB Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), CAD and CPAN and DFF-40 and DFF2 and DFF40, DFFB and IDBG-86952 and ENSG00000169598 and 1677, enzyme binding, nuclei, Dffb and IDBG-206807 and ENSMUSG00000029027 and 13368, BT.22809 and IDBG-634397 and ENSBTAG00000020015 and 512730

Gene info

• Identity
  HGNC:2773
• Gene
  DFFB
• Long gene name
  DNA fragmentation factor subunit beta
• Synonyms gene name
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• Synonyms
  • CAD
  • CPAN
  • DFF-40
  • DFF40
• Synonyms name
  • caspase-activated DNase
• Locus
  1p36.32
• Discovery year
  1997-10-27
• Entrez gene record
  1677
• Pubmed identfication
  • 9108473
  • 9560346
• RefSeq identity
  • NM_001282669
• VEGA ID
  OTTHUMG00000003525

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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