Mouse anti actin alpha-muscle

  • Catalog number
    MUB0107P
  • Price
    Please ask
  • Size
    0,1 mg
  • Category
    Primary Antibodies
  • Long description
    Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Alpha- muscle actin is present in striated as well as smooth muscle cells, and in pathological tissues derived therefrom. It has for example been detected in several types of muscle derived tumors, and also been shown to appear in stress fibers of fibroblastic cells involved in contractile phenomena such as wound healing and fibrocontractive diseases.
  • Antibody come from
    HHF35 is a Mouse monoclonal IgG1 antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with an SDS-extracted protein fraction from Human myocardium.
  • Other description
    Each vial contains 50 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
  • Clone
    HHF35
  • Antigen antibody binding interaction
    Mouse anti actin alpha-muscle Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Chicken,Human,Monkey,Rabbit,Rat,Swine,Zebrafish
  • Antibody s reacts with these species
    The epitope recognized by α-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
  • Antibody s specificity
    HHF35 reacts with both α-muscle and γ-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections.
  • Research interest
    Cardiovascular,Cytoskeleton,Stem cell research
  • Application
    Electron microscopy,ELISA,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
  • Antibody s suited for
    HHF35 is useful for immunohistochemistry on frozen and paraffin-embedded tissues preserved in several types of fixatives (see reference 1), immunoblotting, immuno-electron microscopy and ELISA. For immunohistochemical applications to paraffin embedded tissues it is recommended to dilute the antibody in PBS containing 50 mM EDTA. Optimal antibody dilution should be determined by titration; recommended range is 1:200 – 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 – 1:5000 for immunoblotting applications.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    Tsukada, T., Tippens, D., Gordon, D., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. American Journal of Pathology 126, 51-60.Tsukada, T., McNutt, M.A., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic Human tissues. American Journal of Pathology 127, 389-402.Schmidt, R.A., Cone, R., Haas, J.E. and Gown, A.M. (1988). Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed anti muscle actins. American Journal of Pathology 131, 19-28.Babaev, V.R., Bobryshev, Y.V., Stenina, O.V., Tararak, E.M. and Gabani, G. (1990). Heterogeneity of smooth muscle in atheromatous plaque of Human aorta. American Journal of Pathlogy 136, 1031-42.Nascimento, C., Caroli-Bottino, A., Paschoal, J. and Pannain, V.L. (2009). Vascular immunohistochemical markers: contributions to hepatocellular nodule diagnosis in explanted livers. Transplantation Proceedings 41, 4211-13.
  • Protein number
    P62736 & P68032
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    The Mouse anti actin alpha-muscle is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    ACTC1, ACTA2, CAPZA1
  • Short name
    Mouse anti actin alpha-muscle
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to actin a-muscle
  • Alternative technique
    murine, antibodies
  • Tissue
    muscle
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    capping actin protein of muscle Z-line subunit alpha 1
  • Synonyms gene name
    • capping protein (actin filament) muscle Z-line, alpha 1
    • capping actin protein of muscle Z-line alpha subunit 1
  • GenBank acession
  • Locus
  • Discovery year
    1994-05-26
  • Entrez gene record
    829
  • Pubmed identfication
  • RefSeq identity
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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