MMP-9, Active, human recombinant
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Catalog number7867-500
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PricePlease ask
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Size500 Units
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SynonymsMatrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2
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Alternative_namesMatrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2
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Description>95% Pure, Active, Human Recombinant, MMP-9/Matrix Metallopeptidase 9. A Protein involved in breakdown of extracellular matrix
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test methodThe activity was determined using fluorogenic substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is > 70 U/µg.
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Results> 70 U/µg
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Unit DefinitionOne unit (U) of the activity is defined as the amount of enzyme that releases 1 pmole of Mca from Mca-PLGL-Dpa-AR-NH2 per min. at 37°C.
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Molecular Weight39 kDa (aa 107-457 + NT His Tag)
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from a proprietary buffer
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Reconstitution InstructionsReconstitute with pre-chilled 30% Glycerol solution (in dH2O) to 10 U/µl and incubate on ice until it completely dissolves. Aliquot and store the reconstituted MMP-9 at -20°C. Stable for 2 months after reconstitution.
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Background InformationMatrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B, is the mostly studied MMP, due to its fundamental role in cancer biology, autoimmune disease, and other conditions. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is structurally a multi-domain metalloenzyme, composed of a prodomain, a catalytic domain, a gelatin binding domain, a metal-binding domain, and a carboxyl terminal hemopexin like domain. This active human MMP-9 is composed of the catalytic domain, a gelatin binding domain, and a metal binding domain (AA 107-457). The protein was expressed in E.coli and purified and refolded using proprietary techniques.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMMP9, MMP14, MMP24, MMP28, RNU6-9, PIRC47, PIRC38, PIRC43, PIRC45
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Short nameMMP-9, Active, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameMMP-9, functionnal, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 9
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
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Locus
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Discovery year1990-03-14
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Entrez gene record
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Pubmed identfication
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 14
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Synonyms gene name
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 24
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Synonyms gene name
- matrix metalloproteinase 24 (membrane-inserted)
- matrix metallopeptidase 24 (membrane-inserted)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 28
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Synonyms gene name
- matrix metalloproteinase 28
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 43
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data