MTP1, vacuolar Zn2+/H+ antiporter
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Catalog numberAS09 485
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PricePlease ask
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Size100 µl
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Available ordering formatInquire
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ImmunogenKLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 Q9ZT63, At2g46800
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationsELISA (ELISA), western blot (WB)
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Connected productscollection of antibodies to plant tonoplast membrane proteinsAS09 474 | anti-AtMTP4 | metal tolerance protein BPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent43.8 | 43 kDa (Arabidopsis thaliana)
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Verified reactivityArabidopsis thaliana
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Possible reactivitydicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens,
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No reactivitymembers of Solanaceae
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Manufactured by Operon Biotechnologies.
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ReferencesKawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383. Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.
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Scientific contextMTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1
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Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.MTP1 protein is of low abundance in plant tissues.Protocol for isolation of tonoplast proteins can be found here.
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Protein numberQ9ZT63
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TAIR numberAT2G46800
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Gene target
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Gene symbolSLC40A1
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Short nameMTP1, vacuolar Zn2+/H+ antiporter
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Alternative nameMTP1, vacuolar Zn2+/H+ antiporter
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Gene info
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Identity
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Gene
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Long gene namesolute carrier family 40 member 1
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Synonyms gene
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Synonyms gene name
- solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
- solute carrier family 40 (iron-regulated transporter), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-01-04
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Entrez gene record
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Pubmed identfication
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Classification
- Solute carriers
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data