MTP1, vacuolar Zn2+/H+ antiporter

  • Catalog number
    AS09 485
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Inquire
  • Immunogen
    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 Q9ZT63, At2g46800
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
  • Verified applications
    ELISA (ELISA), western blot (WB)
  • Connected products
    collection of antibodies to plant tonoplast membrane proteinsAS09 474 | anti-AtMTP4 | metal tolerance protein BPlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
  • Molecular weight expected аpparent
    43.8 | 43 kDa (Arabidopsis thaliana)
  • Verified reactivity
    Arabidopsis thaliana
  • Possible reactivity
    dicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens,
  • No reactivity
    members of Solanaceae
  • Supplementary information
    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Manufactured by Operon Biotechnologies.
  • References
    Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383. Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.
  • Scientific context
    MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1
  • Notes
    0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.MTP1 protein is of low abundance in plant tissues.Protocol for isolation of tonoplast proteins can be found here.
  • Protein number
    Q9ZT63
  • TAIR number
    AT2G46800
  • Gene target
  • Gene symbol
    SLC40A1
  • Short name
    MTP1, vacuolar Zn2+/H+ antiporter
  • Alternative name
    MTP1, vacuolar Zn2+/H+ antiporter
Gene info
  • Identity
  • Gene
  • Long gene name
    solute carrier family 40 member 1
  • Synonyms gene
  • Synonyms gene name
    • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
    • solute carrier family 40 (iron-regulated transporter), member 1
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
  • Discovery year
    2000-01-04
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Solute carriers
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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