Goat Anti-Bovine IgG (H+L)-FITC

  • Catalog number
    MBS674799
  • Price
    Please ask
  • Size
    1 mg
  • Products_type
    Secondary Antibody
  • Products_short_name
    [Goat Anti-Bovine IgG (H+L)]
  • Products_name_syn
    [Goat Anti-Bovine IgG(H+L) - FITC; Goat Anti-Bovine IgG (H+L)]
  • Clonality
    Polyclonal
  • Reactivity
    Bovine
  • Specificity
    Reacts with the heavy and light chains of bovine IgG as demonstrated by ELISA.May also react with the light chains of other bovine immunoglobulins.
  • Purity
    Affinity chromatography on bovine IgG covalently linked to agarose.
  • Form
    Fluorescein (FITC) Conjugate
  • Storage_stability
    The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
  • Tested_application
    ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
    H+L  
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    Goat Anti- IgG (H+L)-FITC
  • Technique
    anti-, anti, FITC, Goat, IgG, antibody to, antibodies, Fluorescein, goats, IgGs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Species
    Bovine, Bos Taurus genes are available form the Bovine Genome database. Goats
  • Alternative name
    caprine Antibody toBovine Immunoglobulin G (heavy + light chain)-fluorecein
  • Alternative technique
    antibodies, caprine, fluorescine, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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