Hepatic Steatosis Assay Kit

• Catalog number
  K584-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O.
• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) for Triglycerides and Microscopy for Histochemical staining of Lipid droplets Applications: • Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation • Screen drug candidates for steatotic risk
• Detection Method
  Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) and Microscopy
• Species Reactivity
  Mammalian
• Applications
  • Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation • Screen drug candidates for steatotic risk
• Sample Type
  Liver cells such as HepG2
• Features Benefits
  • Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O. • The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes. • Easy and simple protocol. This kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. • Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2.
• Storage Conditions
  4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Hepatic Steatosis, also known as fatty liver, is a disease characterized by an excessive accumulation of lipid droplets within the liver. This can be caused by multiple factors, including excessive alcohol intake, obesity, and other diseases with abnormal fat metabolism. Hepatic Steatosis is commonly used as a marker to determine the hepatotoxicity of drug candidates. BioVision’s Hepatic Steatosis Kit provides reagents for measurement of intracellular triglyceride accumulation and staining reagents to visualize the lipid droplet formation in hepatocytes. The kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2. The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Hepatic   Steatosis   Kit  
• Short name
  Hepatic Steatosis Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Hepatic Steatosis test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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