Nuclear/Cytosolic Fractionation Kit

Shipping Temp

2 parts: 1 RT / 1 Blue Ice
Storage Temp

Upon receipt, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Detection Method

N/A
Category Product Type

Kit
URL link to datasheet
URL link to SDS

more (AKR-171, AKR-172) GHS.pdf
UNSPSC

41105500
Product Line

Cell Signaling and Protein Biology
Short Description

Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.
Product feature 1

Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells
Product feature 2

Extraction in less than 2 hours
Product feature 3

Each prep can accommodate up to 5 x 106cells

Gene target

Cytosolic Fractionation Kit
Short name

/Cytosolic Fractionation Kit
Alternative name

Nuclear/Cytosolic Fractionation reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832