MHC Class II antibody biotin

• Catalog number
  61R-M110aBT
• Price
  Please ask
• Size
  100 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Immunology
• Type of Immunogen
  MHC Class II antibody (biotin) was raised in mouse using purified class II from T cell line ..
• Raised in
  Mouse
• Specificity
  Human
• Cross Reactivity
  Should be determined by the end user
• Isotypes
  IgG1
• Clone
  TDR31.1
• Conjugate Tag
  Biotin
• Grade Purity
  Grater than 95% pure
• Method of Purification
  MHC Class II beta chain antibody (biotin) was purified by Protein A chromatography.
• Concentration
  1.0 mg/ml
• Form Buffer
  50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  FC; WB
• Usage Recommendations
  FC: 5 ug/ml
• Additional Information
  This antibody recognizes a monomorphic class II beta chain epitope.
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  MHC   Class  
• Gene symbol
  CD74, BTNL2, RFX1
• Short name
  MHC Class II antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  Mouse monoclonal MHC Class II antibody (biotin)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1697
• Gene
  CD74
• Long gene name
  CD74 molecule
• Synonyms gene
  • DHLAG
• Synonyms gene name
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain
• Synonyms
  • CLIP
• Synonyms name
  • HLA-DR-gamma
  • Ia-associated invariant chain
  • gamma chain of class II antigens
  • MHC HLA-DR gamma chain
  • class II MHC-associated invariant chain peptide
• Locus
  5q33.1
• Discovery year
  1986-01-01
• Entrez gene record
  972
• Pubmed identfication
  • 6324166
  • 3001652
• RefSeq identity
  • NM_004355
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000163559

Gene info

• Identity
  HGNC:1142
• Gene
  BTNL2
• Long gene name
  butyrophilin like 2
• Synonyms gene name
  • butyrophilin-like 2 (MHC class II associated)
  • butyrophilin-like 2
• Synonyms
  • HSBLMHC1
  • BTL-II
  • BTN7
• GenBank acession
  • AF186588
• Locus
  6p21.32
• Discovery year
  2000-08-22
• Entrez gene record
  56244
• Pubmed identfication
  • 10803852
  • 15735647
• RefSeq identity
  • NM_019602
• Classification
  • Butyrophilins
  • C2-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000031102

Gene info

• Identity
  HGNC:9982
• Gene
  RFX1
• Long gene name
  regulatory factor X1
• Synonyms gene name
  • regulatory factor X, 1 (influences HLA class II expression)
• Synonyms
  • EF-C
• Synonyms name
  • trans-acting regulatory factor 1
  • enhancer factor C
  • MHC class II regulatory factor RFX
• Locus
  19p13.12
• Discovery year
  1991-11-06
• Entrez gene record
  5989
• Pubmed identfication
  • 1505960
  • 8289803
• RefSeq identity
  • NM_002918
• Classification
  • Regulatory factor X family
• VEGA ID
  OTTHUMG00000181979

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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