Mouse Anti-Human AMIGO-1 Monoclonal IgG1, Clone S86-36

• Catalog number
  SMC-438D-RPE
• Price
  Please ask
• Size
  100 µg

• Clone
  S86-36
• Immunogen
  Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1
• Antibody s full description
  Mouse Anti-Human AMIGO1 Monoclonal IgG1 Antibody, Clone: S86-36: RPE
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  AMIGO 1 Antibody, AMIGO1 Antibody, Adhesion molecule with Ig like domain 1 Antibody, Amphoterin-induced protein 1 Antibody, Alivin-2 Antibody, Alivin 2 Antibody, Ali2 Antibody, AMIGO Antibody, KIAA1163 Antibody, Amphoterin induced gene and ORF (Amigo) Antibody, Amphoterin induced protein 1 Antibody, MGC25558 Antibody, OTTHUMP00000013379 Antibody, RP23 89M15.6 Antibody
• Raised in
  Mouse
• Antibody s target
  AMIGO1
• Primary research fields
  Neuroscience, Cell Structure, Pre-Synaptic Markers
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~60-80kDa depending on maturity/glycosylation.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Projection , Axon
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
• Bibliography
  1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
• Released date
  5-Feb-2013
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_065754.2
• Gene number
  57463
• Protein number
  Q86WK6
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (SMC-438). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (SMC-438) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMIGO-1 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (SMC-438). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (SMC-438) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa. Mouse Anti-AMIGO-1 Antibody [S86-36] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-438) Mouse Anti-AMIGO-1 Antibody [S86-36] used in Western Blot (WB) on Rat Brain Membrane (SMC-438)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Latin name
  Mus musculus

• Gene target
  AMIGO-1   Monoclonal   IgG1   S86-36  
• Gene symbol
  AMIGO1, SNORD115-36, IGKV2D-36, IGHV3-36, IGKV2-36, IGLV1-36, SNORA35, RNU6-36P, PDLIM1, THAP4
• Short name
  Mouse Anti- AMIGO-1 Monoclonal IgG1, Clone S86-36
• Technique
  anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  RPE
• Species
  Human, Humans, Mouses
• Alternative name
  Mouse Antibody toHuman AMIGO-1 monoclonal IgG1, clonality S86-36
• Alternative technique
  murine, antibodies
• Clone name
  S86-36

Gene info

• Identity
  HGNC:20824
• Gene
  AMIGO1
• Long gene name
  adhesion molecule with Ig like domain 1
• Synonyms
  • AMIGO
  • KIAA1163
• Synonyms name
  • amphoterin-induced gene and open reading frame
• Locus
  1p13.3
• Discovery year
  2005-06-23
• Entrez gene record
  57463
• Pubmed identfication
  • 12629050
• RefSeq identity
  • NM_020703
• Classification
  • V-set domain containing
• VEGA ID
  OTTHUMG00000011653

Gene info

• Identity
  HGNC:33055
• Gene
  SNORD115-36
• Long gene name
  small nucleolar RNA, C/D box 115-36
• Synonyms
  • HBII-52-36
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033810
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003351.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5802
• Gene
  IGKV2D-36
• Long gene name
  immunoglobulin kappa variable 2D-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2D-36
  • immunoglobulin kappa variable 2D-36 pseudogene
• GenBank acession
  • X71893
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28880
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151611

Gene info

• Identity
  HGNC:5599
• Gene
  IGHV3-36
• Long gene name
  immunoglobulin heavy variable 3-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-36
  • immunoglobulin heavy variable 3-36 pseudogene
• GenBank acession
  • M99667
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28431
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152068

Gene info

• Identity
  HGNC:5786
• Gene
  IGKV2-36
• Long gene name
  immunoglobulin kappa variable 2-36 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2-36
  • immunoglobulin kappa variable 2-36 pseudogene
• GenBank acession
  • X71889
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28918
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151683

Gene info

• Identity
  HGNC:5876
• Gene
  IGLV1-36
• Long gene name
  immunoglobulin lambda variable 1-36
• GenBank acession
  • Z73653
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28826
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151074

Gene info

• Identity
  HGNC:32625
• Gene
  SNORA35
• Long gene name
  small nucleolar RNA, H/ACA box 35
• Synonyms
  • HBI-36
  • SNORA35A
• Locus
  Xq23
• Discovery year
  2006-04-04
• Entrez gene record
  677816
• Pubmed identfication
  • 15199136
  • 16381836
• RefSeq identity
  • NR_002993
• Classification
  • Small nucleolar RNAs, H/ACA box

Gene info

• Identity
  HGNC:34280
• Gene
  RNU6-36P
• Long gene name
  RNA, U6 small nuclear 36, pseudogene
• Synonyms gene
  • RNU6-36
• Synonyms gene name
  • RNA, U6 small nuclear 36
• Locus
  12q23.1
• Discovery year
  2008-06-10
• Entrez gene record
  100873757
• RefSeq identity
  • NR_046494

Gene info

• Identity
  HGNC:2067
• Gene
  PDLIM1
• Long gene name
  PDZ and LIM domain 1
• Synonyms gene
  • CLIM1
• Synonyms gene name
  • PDZ and LIM domain 1 (elfin)
• Synonyms
  • CLP-36
  • hCLIM1
  • CLP36
• Synonyms name
  • carboxyl terminal LIM domain protein 1
  • elfin
• GenBank acession
  • U90878
• Locus
  10q23.33
• Discovery year
  1998-01-21
• Entrez gene record
  9124
• Pubmed identfication
  • 10861853
• Classification
  • LIM domain containing
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000018810

Gene info

• Identity
  HGNC:23187
• Gene
  THAP4
• Long gene name
  THAP domain containing 4
• Synonyms
  • CGI-36
  • Nb(III)
• Synonyms name
  • nitrobindin
• GenBank acession
  • AF258556
• Locus
  2q37.3
• Discovery year
  2003-10-08
• Entrez gene record
  51078
• Pubmed identfication
  • 12575992
  • 10810093
  • 30524950
• RefSeq identity
  • NM_015963
• Classification
  • THAP domain containing
• VEGA ID
  OTTHUMG00000133410

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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