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Category
Primary Antibodies
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Long description
The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is repliCated, a process that can be detected using biochemical techniques, such as the BrdU incorpoRation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA repliCation, and G2-phase, the gap between completion of DNA repliCation and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.
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Antibody come from
8B3G is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a total cell lysate of the Human bladder carcinoma cell line T24.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
8B3G
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Antigen antibody binding interaction
Mouse anti Mitotic cells Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human,Zebrafish
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
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Research interest
Cell cycle
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Application
Flow Cytometry,Immunocytochemistry
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Antibody s suited for
8B3G can be used for flow cytometric analyses and immunocytochemistry. 8B3G is not suitable for immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:10 for flow cytometry, and for immunocytochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
1. No references available
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
