CBX3 Antibody

• Catalog number
  6147-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  CBX3
• Target Alternative Name
  HECH, HP1­GAMMA, HP1Hs­gamma, HP1γ
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Fusion protein of human CBX3
• Accession
  Q13185
• Gene Id
  11335
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot, Immunohistochemistry, Immunofluorescene
• Positive Control
  Western Blot: Various cell lysates
• Application And Usages
  Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:100, IF: 1:20 – 1:50
• Description
  Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; However, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino terminal chromodomain, followed by a variable hinge region and a conserved carboxy­terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri­methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins  implicated in gene silencing and heterochromatin  formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA  methyltransferases, and the p150 subunit of chromatin­assembly factor­1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post­translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CBX3  
• Gene symbol
  CBX3
• Short name
  CBX3 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  CBX3 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1553
• Gene
  CBX3
• Long gene name
  chromobox 3
• Synonyms gene name
  • chromobox homolog 3 (Drosophila HP1 gamma)
  • chromobox homolog 3
• Synonyms
  • HP1Hs-gamma
• Synonyms name
  • HP1 gamma homolog (Drosophila)
• GenBank acession
  • U26312
• Locus
  7p15.2
• Discovery year
  1999-10-12
• Entrez gene record
  11335
• Pubmed identfication
  • 8663349
• RefSeq identity
  • NM_007276
• Classification
  • Chromobox family
• VEGA ID
  OTTHUMG00000022911

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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