Mouse Anti-Human Kir2.3 Monoclonal IgG1, Clone S25-35

  • Catalog number
    SMC-312D-P594
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S25-35
  • Immunogen
    Fusion protein amino acids 390-445 of human Kir2.3
  • Antibody s full description
    Mouse Anti-Human Kir2.3 Monoclonal IgG1 Antibody, Clone: S25-35: PE/ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    IRK3 Antibody, HIR Antibody, HIRK2 Antibody, HRK1 Antibody, KCNJ4 Antibody, potassium inwardly rectifying channel subfamily J member 4 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Kir2.3
  • Primary research fields
    Neuroscience, Ion Channels, Potassium Channels, Inward-Rectifying Potassium Channels
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~45kDa. No cross-reactivity against Kir2.1 or Kir2.2.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-312 was sufficient for detection of Kir2.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane , Cytoplasmic Vesicle Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene (1-3).
  • Bibliography
    1. Zobel C., et al. (2003) J Physiol. 550: 365-372. 2. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 3. Munoz V., et al. (2007) Heart Rhythm. 4(4): 487-496.
  • Released date
    6-Jul-2009
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_004972.1
  • Gene number
    3761
  • Protein number
    P48050
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody, Clone S25-35 (SMC-312). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody (SMC-312) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of Kir2.3 Potassium Channel protein using Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody, Clone S25-35 (SMC-312). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody (SMC-312) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Kir2.3 Potassium Channel Antibody [S25-35] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-312) Mouse Anti-Kir2.3 Potassium Channel Antibody [S25-35] used in Western Blot (WB) on Human Cell lysates (SMC-312)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
    Kir2   Monoclonal   IgG1   S25-35  
  • Gene symbol
    RPS25, IGLV7-35, SNORD115-35, IGKV1-35, IGHV3-35, IGKV1D-35, RNU6-35P, MRPS25, PNPT1, PHF2
  • Short name
    Mouse Anti- Kir2 3 Monoclonal IgG1, Clone S25-35
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    PE/ATTO 594
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman Kir2.3 monoclonal IgG1, clonality S25-35
  • Alternative technique
    murine, antibodies
  • Clone name
    S25-35
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable 7-35 (pseudogene)
  • Synonyms gene name
    • immunoglobulin lambda variable 7-35
    • immunoglobulin lambda variable 7-35 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 1-35 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 1-35
    • immunoglobulin kappa variable 1-35 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 3-35 (non-functional)
  • Synonyms gene name
    • immunoglobulin heavy variable 3-35
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 1D-35 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 1D-35
    • immunoglobulin kappa variable 1D-35 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 35, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 35
  • Locus
  • Discovery year
    2008-06-10
  • Entrez gene record
  • RefSeq identity
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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