APRIL, Human Recombinant

  • Catalog number
    7117-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    A Proliferating-inducing Ligand, TNFSF13, TRDL-1α
  • Alternative_names
    A Proliferating-inducing Ligand, TNFSF13, TRDL-1α
  • Description
    Stimulates proliferation of various tumor cell lines like Jurkat T cells and MCF-7 carcinoma cells.
  • Recombinant
    Yes
  • Source
    Hi-5 insect cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Endotoxin Level
    < 0.1 ng/ug of protein (
  • Activity Specifications test method
    Determined by its ability to induce cell death in Jurkat cells.
  • Biological activity
    Determined by its ability to induce cell death in Jurkat cells.
  • Molecular Weight
    16.3 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5, 500 mM NaCl and 5% Trehalose.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
  • Background Information
    APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.
  • Amino acid sequence
    AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    APRIL  
  • Gene symbol
    TNFSF13, ANP32B
  • Short name
    APRIL, Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    APRIL, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
  • Identity
  • Gene
  • Long gene name
    TNF superfamily member 13
  • Synonyms gene name
    • tumor necrosis factor (ligand) superfamily, member 13
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-12-04
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Tumor necrosis factor superfamily
    • CD molecules
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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