APRIL, Human Recombinant
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Catalog number7117-10
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PricePlease ask
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Size10 ug
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SynonymsA Proliferating-inducing Ligand, TNFSF13, TRDL-1α
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Alternative_namesA Proliferating-inducing Ligand, TNFSF13, TRDL-1α
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DescriptionStimulates proliferation of various tumor cell lines like Jurkat T cells and MCF-7 carcinoma cells.
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RecombinantYes
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SourceHi-5 insect cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Activity Specifications test methodDetermined by its ability to induce cell death in Jurkat cells.
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Biological activityDetermined by its ability to induce cell death in Jurkat cells.
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Molecular Weight16.3 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5, 500 mM NaCl and 5% Trehalose.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationAPRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.
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Amino acid sequenceAVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolTNFSF13, ANP32B
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Short nameAPRIL, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameAPRIL, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameTNF superfamily member 13
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 13
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameacidic nuclear phosphoprotein 32 family member B
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Synonyms gene name
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-02-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ANP32 acidic nuclear phosphoproteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data