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Synonyms
SCARB2, CD36L2, LIMP2, LIMPII, LGP85, CD36, AMRF, EPM4, HLGP85, SR-BII
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Alternative_names
SCARB2, CD36L2, LIMP2, LIMPII, LGP85, CD36, AMRF, EPM4, HLGP85, SR-BII
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Description
≥95% pure, active, human cell expressed, human recombinant SCARB2 /CD36L2 /LIMP2 - a lysosomal receptor for glucosylceramidase (GBA) targeting
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh SCARB2 / CD36L2/ LIMP2 Fc Chimera at 5 µg/ml (100 µl/well) can bind rhTSP-2/His with a linear range of 0.1 - 5 µg/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized rh SCARB2 / CD36L2/ LIMP2 Fc Chimera at 5 µg/ml (100 µl/well) can bind rhTSP-2/His with a linear range of 0.1 - 5 µg/ml.
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Molecular Weight
This protein fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 72.5 kDa. The predicted N-terminus is Arg 27. DTT-reduced Protein migrates as 90-115 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
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Background Information
Scavenger receptor class B member 2 (SCARB2) is also known as Lysosome membrane protein 2 (LIMP2), 85 kDa lysosomal membrane sialoglycoprotein (LGP85), CD36 antigen-like 2 (CD36L2, LIMP2), which belongs to the CD36 family. SCARB2 acts as a lysosomal receptor for glucosylceramidase (GBA) targeting. It may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMP2 enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMP2 also has been shown to bind thrombospondin-1, may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
SCARB2
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Short name
CellExp™ SCARB2 /CD36L2 /LIMP2, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ scavenger receptor class B, member 2 /CD36L2 /LIMP2, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
scavenger receptor class B, member 2, AMRF and CD36L2 and EPM4 and HLGP85 and LGP85 and LIMP-2 and LIMPII and SR-BII, SCARB2 and IDBG-25361 and ENSG00000138760 and 950, enzyme binding, Plasma membranes, Scarb2 and IDBG-180164 and ENSMUSG00000029426 and 12492, SCARB2 and IDBG-643116 and ENSBTAG00000010956 and 533177
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