-
Availability
Please contact us to check the availability
-
Immunogen
Urea extract of human hair
-
Specificity
44-46 kDa acid hard keratin,
-
Species reactivity
Human, mouse, rabbit, sheep
-
Product presentation
Protein G purified antibody in PBS containing 0.1% sodium azide
-
Product category
Primary Antibody
-
Antibody s raised in
Mouse
-
Antibody s isotype
Ig
-
Antibody s clone
AE13
-
Product is supplied as
liquid concentrate
-
Recommended concentration for use
WB 1:500-1:1500
-
Protein concentration
100 µl
-
Positive control
Human hair (cuticle and cortex)
-
Pretreatment
WB: Extract of human hair with 8M urea, 200 mM TRIS/HCl pH 9.5 and 200 mM 2-Mercaptoethanol. IHC: Unmasking of paraffin sections has not been established, the use of unmasking fluid G (Art. No. DE007) analogue to CK5, CK8 and CK14 may be advantageous.
-
Tested applications
IHC(C, P), WB
-
Recommended secondary reagent
Anti-Mouse IgG-conjugate, biotinylated (Art. Nr. ZU102 or ZU122)
-
Storage method
Store at 2-8°C
-
UniProt number
Go to UniProt/SwissProt
-
Litterature
1. Lynch M.H., O'Guin W.M., Hardy C., Mak L., and Sun T-T. (1986) Acidic and basic hair/nail ("hard") keratins: Their localization in upper cortical and cuticle cells of the human hair follicle and their relationship to "soft" keratins. The Journal of Cell Biology 103(6) 2593-2606. 2. Dhouailly, D., Xu, C., Manabe, M., Schermer, A., and Sun, T.-T. (1989). Expression of hair related keratins in a soft epithelium: subpopulations of human and mouse dorsal tongue keratinocytes express keratin markers for hairskin- and esophageal-types of differentiation. Experimental Cell Research 181, 141-158.
-
Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
