Target of this antibody:
Alternative names of antibody target:
Kruppel-like factor 4, GKLF, EZF
KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development sμggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
Clonality of antibody:
This antibody reacts with:
Human, Mouse, Rat
KLF4 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (1 mg/ml) in 1X PBS containing 0.02 % sodium azide.
Affinity chromatography purified via peptide column
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA
Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
anti-KLF4 mAntibody (4G6E11)
antibody to-KLF4 mAntibody (4G6E11)