KLF4 mAntibody (4G6E11)

• Catalog number
  5300-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  KLF4
• Target Alternative Name
  Kruppel-like factor 4, GKLF, EZF
• Antibody Type
  Monoclonal
• Clone
  4G6E11
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  KLF4 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.
• Accession
  O43474
• Gene Id
  9314
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg (1 mg/ml) in 1X PBS containing 0.02 % sodium azide.
• Purification
  Affinity chromatography purified via peptide column
• Application
  Western blot, ELISA
• Positive Control
  Western Blot: Mouse liver tissue lysate
• Application And Usages
  Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
• Description
  KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development sμggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link

• Gene target
  KLF4   mAntibody   4G6E11  
• Gene symbol
  KLF4
• Short name
  KLF4 mAntibody (4G6E11)
• Isotype
  Mouse IgG1
• Alternative name
  KLF4 mAntibody (4G6E11)

Gene info

• Identity
  HGNC:6348
• Gene
  KLF4
• Long gene name
  Kruppel like factor 4
• Synonyms gene name
  • Kruppel-like factor 4 (gut)
• Synonyms
  • EZF
  • GKLF
• Synonyms name
  • gut Kruppel-like factor
• GenBank acession
  • AF022184
• Locus
  9q31.2
• Discovery year
  1999-12-14
• Entrez gene record
  9314
• Pubmed identfication
  • 9422764
  • 16372018
• RefSeq identity
  • NM_004235
• Classification
  • Zinc fingers C2H2-type
  • Kruppel like factors
• VEGA ID
  OTTHUMG00000020449

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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