GST, tag

• Catalog number
  AS15 2861
• Price
  Please ask
• Size
  50 µg

• Available ordering format
  Lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
• Immunogen
  GST protein
• Raised in
  Mouse
• Clonality
  Monoclonal
• Clone
  IgG2a, clone GST.B6
• Purification
  Affinity purified
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipitation (IP), immunohistochemistry (IHC)
• Connected products
  collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1 : 5000 (0.2 µg/µl) with ECL (WB)
• Molecular weight expected аpparent
  26 kDa
• Verified reactivity
  native and denatured forms of purified GST or GST fusion proteins
• Possible reactivity
  native and denatured forms of purified GST or GST fusion proteins
• No reactivity
  no confirmed exceptions from predicted reactivity are currently known
• Supplementary information
  Antibody is purified on Protein A
• References
  to be added when available antibody available in March 2015
• Scientific context
  GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
• Notes
  Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  GST   tag  
• Gene symbol
  MGST3
• Short name
  GST, tag
• Technique
  Tags
• Label
  tag
• Alternative name
  GST, detection labelled

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

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