OxiSelect™ BPDE Protein Adduct ELISA test kit

  • Catalog number
    STA-301
  • Price
    Please ask
  • Size
    96 assays
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
  • Technical file
    more kit.pdf
  • SDS or safty data sheet
    details (STA-301) GHS.pdf
  • Type of reagent
    Oxidative Stress/Damage
  • Related techniques
    Oxidative Stress/ Damage, Protein Oxidation / Nitration, BPDE Protein Adduct ELISA
  • Use of the reagent
    BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC.  The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody.  The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
  • Cellular use
    For detection and quantification of benzo(a)pyrene diol epoxide (BPDE) protein adducts
  • Protocol information
    BPDE-BSA standard included
  • Reagent properties
    Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,23
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Storage
    Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
  • Group
    Enzyme-Linked Immunosorbent Assay kits
  • Gene target
  • Short name
    OxiSelect™ BPDE Protein Adduct ELISA test kit
  • Technique
    ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    OxiSelect™ BPDE Protein Adduct Enzyme-linked immunosorbent assay test reagent
  • Alternative technique
    elisas, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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