M-MulV Reverse Transcriptase, 25000 u.a.
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Catalog number
E318-25000
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Price
Please ask
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Size
25000 u.a.
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Enzyme prototype
N/A
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Ordering
To order the M-MulV Reverse Transcriptase, 25000 u.a., please use the Cat. #E318-25000 and send by email or Fax your purchase order. The Purchase Order must include shipping and billing addresses, applicable VAT number, desired quantity.
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Contact
Prices are indicative. Gentaur/Genprice can alter prices without further notice. Please contact us for more information about the latest availability and price.
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Technical file
Please contact our technical support at +32 16 58 90 45 to receive the latest datasheet and certificate of analysis
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Precautions
The M-MulV Reverse Transcriptase, 25000 u.a. is manufactured in Europe and is intended to be used for research use only, not for diagnostics purposes. Do not use for human or animal consumption.
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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