Synonyms
Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.
Alternative_names
Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.
Description
A serine protease which activates zymogen
Recombinant
No
Source
Human urine
Purity by SDS-PAGE
≥90%
Assay
SDS-PAGE
Purity
≥90%
Activity (Specifications/test method)
1 nM UK will cause a change in absorbance of 0.001 at 405 nm in 1 minute at R/T in 100 µl reaction mixture containing 0.05 M Tris-HCl, 0.1 M NaCl, pH 7.4, using S2444 (0.6mM) as the substrate.
Molecular Weight
54.0 kDa
Storage Temp.
-20°C
Shipping
Gel pack
Shelf Life
12 months
Appearance
Lyophilized protein
Physical form description
Lyophilized powder
Reconstitution Instructions
Reconstitute in sterile H₂O to a concentration >100 ug/ml. The solution can then be diluted into other aqueous solutions.
Background Information
Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. UK is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54,000.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
