Rabbit Anti-Human MAPK4 (pSer186) Polyclonal: RPE
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Catalog numberSPC-976D-RPE
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PricePlease ask
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser186 of human MAPK4 (AA183-190)
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Antibody s targetHuman MAPK4 (pSer186)
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Antibody s full descriptionRabbit Anti-Human MAPK4 (pSer186) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameERK4 Antibody, ERK3 Antibody, ERK3 related Antibody, MAPK4 Antibody, Extracellular signal regulated kinase 4 Antibody, MAP kinase 4 Antibody, MAP kinase isoform p63 Antibody, MAPK4 Antibody, Mitogen activated protein kinase 4 Antibody, MK04_HUMAN Antibody, p63MAPK Antibody, PRKM4 Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 62.623 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-976 was sufficient for detection of MAPK4 (pSer186) in 10 µg of mouse brain lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificityHighly expressed in heart and brain.
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Scientific contextMAPK4 is a protein-serine/threonine kinase that phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. MAPK4 may promote entry in the cell cycle.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_002738
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Gene number5596
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Protein numberP31152
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolRPE, MAPK4
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Short nameRabbit Anti- MAPK4 (pSer186) Polyclonal: RPE
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelRPE
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman MAPK4 (pSer186) polyclonal: RPE
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase 4
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data