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Synonyms
Endoproteinase Lys/Arg-Arg, Kexin, 3.4.21.61, Protease KEX2, Proteinase YSCF, KEX2, QDS1, YNL238W, N1122
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Alternative_names
Endoproteinase Lys/Arg-Arg, Kexin, 3.4.21.61, Protease KEX2, Proteinase YSCF, KEX2, QDS1, YNL238W, N1122
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Description
A protease Enzyme hydrolysing amide bonds in proteins & peptides.
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Recombinant
Yes
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Source
(BTI-Tn-5B1-4) Hi-5 Insect cells
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Purity by SDS PAGE
≥ 95%
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Assay
SDS-PAGE
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Purity
≥ 95%
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Biological activity
Recombinant Kex-2 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX-2. 1 milligram of recombinant KEX-2 contains activity equivalent to at least 40 units of yeast derived KEX-2. Cleaves at the carboxyl side of K/R-R.
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Molecular Weight
60.4 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Reconstitution Instructions
DI water (~1 mg/ml)
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Background Information
Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Kex-2 cleaves at the carboxyl end of the recognition sequences Arg-Arg/X and Lys-Arg/X. Recombinant Yeast Kex-2 is a 60.4 kDa protease consisting of 558 amino acid residues.
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Amino acid sequence
LPVPAPPMD SSLLPVKEA EDKLSINDP LFERQWHLV NPSFPGSDI NVLDLWYNN ITGAGVVAA IVDDGLDYE NEDLKDNFC AEGSWDFND NTNLPKPRL SDDYHGTRC AGEIAAKKG NNFCGVGVG YNAKISGIR ILSGDITTE DEAASLIYG LDVNDIYSC SWGPADDGR HLQGPSDLV KKALVKGVT EGRDSKGAI YVFASGNGG TRGDNCNYD GYTNSIYSI TIGAIDHKD LHPPYSEGC SAVMAVTYS SGSGEYIHS SDINGRCSN SHGGTSAAA PLAAGVYTL LLEANPNLT WRDVQYLSI LSAVGLEKN ADGDWRDSA MGKKYSHRY GFGKIDAHK LIEMSKTWE NVNAQTWFY LPTLYVSQS TNSTEETLE SVITISEKS LQDANFKRI EHVTVTVDI DTEIRGTTT VDLISPAGI ISNLGVVRP RDVSSEGFK DWTFMSVAH WGENGVGDW KIKVKTTEN GHRIDFHSW RLKLFGESI DSSKTETFV FGNDKEEVE PAATESTVS QYSASSTSI SISATSTSS ISIGVETSA IPQTTTAST DPDSDPNTP
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional source
Recombinants or rec. proteins
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Group
recombinants