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Category
Primary Antibodies
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Long description
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification._x000B__x000B_Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of the ovary, lung and breast but not colon or gastrointestinal tract.
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Antibody come from
n/a
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Other description
Provided as a sterile solution in 50 mM HEPES containing 2 mM EDTA, 100 mM NaCl and 1 mM MgCl2 (pH 7.5). Protein A/G Chromatography
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Clone
C35
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Antigen antibody binding interaction
Mouse anti Keratin 7 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunohistochemistry (Frozen Sections)
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Antibody s suited for
Membrane preparation for use in [35S]GTP S binding assays using lysophosphatidic acid as the ligand for the receptor. Prepared from lysed RH7777 cells and contains purified membrane protein with G-protein coupled EDG7 receptors. NOTE: The protocol below has been condensed. Please see Ref. 5 for more detailed information.
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Storage
-20ºC
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Relevant references
1. Bandoh, K., et al. Molecular cloning and characterization of a novel human G-protein coupled receptor, EDG7, for lysophosphatidic acid. J. Biol. Chem. 1999, 274, 27776-27785_x000B__x000B_2. Im, D.S., et al. Molecular cloning and characterization of a lysophosphatidic acid receptor, Edg-7, expressed in prostate. Mol. Pharmacol. 2000, 57, 753-759_x000B__x000B_3. Fang, X., et al. Lysophospholipid growth factors in the initiation, progression, metastases, and management of ovarian cancer. Ann. N.Y. Acad. Sci. 2000, 905, 188-208_x000B__x000B_4. Zheng, Y., et al. 'Lysophosphatidic acid receptor-selection effects on Jurkat T cell migration through a matrigel model basement membrane.' J. Immunol. 2001, 166, 2317-2322_x000B__x000B_5. Parrill, A.L., et al. 'Identification of Edg1 receptor residues that recognize sphingosine 1-phosphate.' J. Biol. Chem. 2000, 275, 39379-39384.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
