Mouse anti Keratin 13 & 10

  • Catalog number
    X1252M
  • Price
    Please ask
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification._x000B__x000B_This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. These proteins correspond to CK numbers 13 and 10, respectively, according to Moll's classification1. Using formalin-fixed, paraffin-embedded tissue sections, this antibody detects only the CK13. With frozen sections, this antibody serves as a differentation-related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    DE-K13
  • Antigen antibody binding interaction
    Mouse anti Keratin 13 & 10 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)
  • Antibody s suited for
    Detects proenzyme form of human Caspase-7 by Western blot. Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16_x000B__x000B_2. Chandler, J.M., et al. Different subcellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver. J. Bio. Chem. 1998, 273, 10815-10818_x000B__x000B_3. Behrensdorf, H.A., et al. The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7. FEBS Lett. 2000, 466, 143-147
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    Keratin  
  • Gene symbol
    KRTAP10-13P
  • Short name
    Mouse anti Keratin 13 & 10
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to Keratin 13 & 10
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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