Taq DNA Ligase - 10,000 units

Description

Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. Taq DNA Ligase is active at elevated temperatures (45°C-70°C) Product Includes Taq DNA Ligase 10X Taq DNA Ligase Buffer with NAD+
Shipping Condition

Ships with Dry Ice

Gene target

Taq DNA Ligase 000 units
Gene symbol

PRKDC, HLA-DOA
Short name

Taq DNA Ligase - 10 000 units
Technique

dna, Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Intact Genomics TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
Alternative name

Taq Desoxyribonucleic acid Ligase - 10,000 units

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data