Biotin-LC-NHS

• Catalog number
  2345-250
• Price
  Please ask
• Size
  250 mg

• Alternative_name
  Biotin-6-aminohexanoic acid-N-hydroxysuccinimide ester; Biotin-X-NHS
• Description
  Biotin-LC-NHS is a useful reagent for biotinylation of primary amines under alkaline conditions (pH~8-9). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 6-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance.
• CAS Number
  72040-63-2
• Structure Available
  Yes
• Salt Form
  No
• Molecular Formula
  C₂₀H₃₀N₄O₆S
• Molecular Weight
  454.54
• Cell Permeable
  No
• Purity
  ≥95% by HPLC
• Solubilities
  DMSO (20 mg/ml) or DMF (20 mg/ml)
• Handling
  Protect from air and moisture
• Tag Line
  A biotinylation agent for primary amines
• Storage Condition
  -20 ̊C
• Shipping Condition
  gel pack
• Shelf Life
  36 months
• MDL Number
  MFCD00065502
• PubChem CID
  83874
• PubChem CID Link
• SMILES
  C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
• InChi
  InChI=1S/C20H30N4O6S/c25-15(7-4-3-6-14-19-13(12-31-14)22-20(29)23-19)21-11-5-1-2-8-18(28)30-24-16(26)9-10-17(24)27/h13-14,19H,1-12H2,(H,21,25)(H2,22,23,29)/t13-,14-,19-/m0/s1
• InChi Key
  UVGHPGOONBRLCX-NJSLBKSFSA-N
• Data Sheet Link
• MSDS Available
  Yes
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  LC-NHS  
• Gene symbol
  NHS, NHS-AS1, PTPN7, CT83
• Short name
  -LC-NHS
• Label
  Biotin
• Alternative name
  biotinilated-LC-NHS

Gene info

• Identity
  HGNC:7820
• Gene
  NHS
• Long gene name
  NHS actin remodeling regulator
• Synonyms gene name
  • Nance-Horan syndrome (congenital cataracts and dental anomalies)
• Locus
  Xp22.2-p22.13
• Discovery year
  1989-06-30
• Entrez gene record
  4810
• Pubmed identfication
  • 20332100
• RefSeq identity
  • NM_198270
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000022799
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:40403
• Gene
  NHS-AS1
• Long gene name
  NHS antisense RNA 1
• Synonyms gene name
  • NHS antisense RNA 1 (non-protein coding)
• Locus
  Xp22.13
• Discovery year
  2011-07-29
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000021203

Gene info

• Identity
  HGNC:9659
• Gene
  PTPN7
• Long gene name
  protein tyrosine phosphatase non-receptor type 7
• Synonyms
  • HEPTP
  • LC-PTP
• GenBank acession
  • BC001746
• Locus
  1q32.1
• Discovery year
  1993-03-03
• Entrez gene record
  5778
• Pubmed identfication
  • 1510684
• RefSeq identity
  • NM_002832
• Classification
  • Protein tyrosine phosphatases non-receptor type
• VEGA ID
  OTTHUMG00000035931

Gene info

• Identity
  HGNC:33494
• Gene
  CT83
• Long gene name
  cancer/testis antigen 83
• Synonyms gene
  • CXorf61
• Synonyms gene name
  • chromosome X open reading frame 61
• Synonyms
  • KK-LC-1
  • FLJ20611
  • FLJ22913
• Synonyms name
  • Kita-kyushu lung cancer antigen 1
• GenBank acession
  • BC062223
• Locus
  Xq23
• Discovery year
  2007-06-20
• Entrez gene record
  203413
• Pubmed identfication
  • 16651449
• RefSeq identity
  • NM_001017978
• VEGA ID
  OTTHUMG00000022244

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