IGF-BP2, human recombinant

• Catalog number
  7165-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  IBP-2
• Alternative_names
  IBP-2
• Description
  Inhibits IGF-II action in human breast and ovarian carcinoma cells
• Recombinant
  Yes
• Source
  (BTI-Tn-5B1-4) Hi-5 Insect cells
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.
• Biological activity
  Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.
• Molecular Weight
  31.5 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP2 is a cysteine-rich secreted protein produced by bone cells, and is most abundant in the brain. IGF-BP2 has been shown to inhibit IGF-II action in human breast and ovarian carcinoma cells. Recombinant human IGF-BP2 is a 31.5 kDa protein consisting of 289 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain.
• Amino acid sequence
  EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional description
  IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BP2  
• Gene symbol
  SIGLEC5, RIMBP2
• Short name
  IGF-BP2, recombinant
• Technique
  Recombinant, IGF, igfs, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  IGF-BP2, H. sapiens Rec.
• Alternative technique
  rec, igfs

Gene info

• Identity
  HGNC:10874
• Gene
  SIGLEC5
• Long gene name
  sialic acid binding Ig like lectin 5
• Synonyms gene
  • CD33L2
• Synonyms gene name
  • sialic acid binding Ig-like lectin 5
• Synonyms
  • OB-BP2
  • SIGLEC-5
  • CD170
• GenBank acession
  • U71383
• Locus
  19q13.41
• Discovery year
  1998-12-16
• Entrez gene record
  8778
• Pubmed identfication
  • 10343116
• RefSeq identity
  • NM_003830
• Classification
  • CD molecules
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000165510

Gene info

• Identity
  HGNC:30339
• Gene
  RIMBP2
• Long gene name
  RIMS binding protein 2
• Synonyms
  • KIAA0318
  • RBP2
  • MGC15831
  • RIM-BP2
  • PPP1R133
• Synonyms name
  • protein phosphatase 1, regulatory subunit 133
• GenBank acession
  • AB002316
• Locus
  12q24.33
• Discovery year
  2006-01-24
• Entrez gene record
  23504
• Pubmed identfication
  • 10748113
• RefSeq identity
  • NM_015347
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000168385

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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